This protocol provides information on preparing the artificial diet with varying amounts of cannabidiol (CBD), setting up a cultivation environment, and monitoring their physiological and behavioral changes in response to CBD treatment. The authors suggest the tobacco hornworm Manduca sexta as a novel insect model system. However, many difficulties remain in mammalian research, which necessitates the development of non-mammalian model organisms for cannabinoid research. With increased attention on cannabinoids in medicine, several mammalian model organisms have been used to elucidate their unknown pharmaceutical functions.
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